Analysis Note

Positive ControlDaudi cells

Application

ELISA (see comments)r>Gel Shift (see comments)r>Immunoblotting (1:500, chemiluminescence)r>Immunoprecipitation (see comments)

General description

Recognizes the ~65 kDa NF-κB p65 (RelA) protein in Daudi cells.

Rabbit polyclonal antibody. Recognizes the ~65 kDa NF-κB p65 (RelA) protein.

This Anti-NF-κB (p65, RelA) (Ab-2) Rabbit pAb is validated for use in ELISA, Gel Shift, Immunoblotting, Immunoprecipitation for the detection of NF-κB (p65, RelA) (Ab-2).

Immunogen

a synthetic peptide corresponding to amino acids near the N-terminal domain of human NF-κB p65 (RelA), conjugated to KLH

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Kolberg, R., 1997. J. NIH Research9, 29.Van Antwerp, D.J. et al. 1996. Science274, 787.Beg, A.A., et al. 1995. Nature376, 167.Baeuerle, P.A., and Henkel, T., 1994. Ann. Rev. Immunol.12, 141.Wulczyn, F.G., et al. 1992. Nature358, 597.

This antibody has also been reported to work for ELISA, gel shift, and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.

Packaging

100 µL in Plastic ampoule

Physical form

In 5 mM sodium phosphate buffer, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)